Thin layer chromatography developer - examples of dye applications

1. The disperse dye of the disperse dye azo type is a silica gel plate, and the bismuth type is easy to use alumina.
1 silica gel G chloroform: acetone = 9: 1
2 silica gel G benzene: acetone = 9: 1
3 silica gel G toluene: acetic acid = 85: 15
4 silica gel G benzene: chloroform: acetone = 5: 2: 1
5 silica gel G toluene: acetone = 20: 1

2. Cationic dye 1 benzene: toluene = 9: 1 silica gel G
2 chloroform:methanol=8:1 silica gel G
3 silica gel G n-butanol: ethanol: water = 9:1 1:1
4 silica gel G butanol: acetic acid: water = 4: 1 : 5
5 silica gel G butanone: acetic acid: isopropanol = 2: 2: 1
6 silica gel G propanol: formic acid = 8: 2 oxazepine

3. Acid dye 1 Silica gel G n-butanol: ethyl acetate: water = 6 : 1 : 3
2 silica gel G n-butanol: pyridine: water: ammonia = 4: 5: 4: 1
3 silica gel G ethyl acetate: pyridine: water = 6: 3: 1
4BaSO4 DMF: water = 3: 4
5 silica butanol: ammonia = 9: 1

4. Reactive dye 1 silica gel butanol: acetic acid: water = 16: 5: 15 (16: 14: 9)
2 silica butanol: water: DMF = 11 : 11 : 3
3 silica gel G n-propanol: ethyl acetate: water = 6 : 1 : 3
4 silica gel G n-butanol: pyridine: water: ammonia (concentration) = 5:5:3:2 (4:5:4:1)
5 silica gel G pyridine: n-pentanol: ammonia (concentration) = 1.1 1:1 1:1

5. Direct dye 1 alumina acetic acid: water (various ratios)
2 silica gel G butyl acetate: pyridine: water = 30: 45: 25
Alumina G
3 silica gel G n-propanol: ammonia = 2: 1
4 silica gel G n-butanol: water: ammonia = 2: 1 : 1 (upper layer)

6. Metal complex dye 1 Silica gel G Benzene: Acetic acid = 4:1
2 silica gel G chloroform: acetic acid = 9:1
3 polyamide methanol: water: ammonia (concentration) = 80: 16: 4
4 polyamide methanol: ammonia water (concentration) = 95 : 5
5 silica gel G n-butanol: absolute ethanol: 2N ammonia = 60: 20: 20

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