Artificial breeding of allogynogenetic crucian carp (middle)

2 Induction
2.1 Mastering the ovulation period The breeding season of the silver puffer varies from place to place. The Yangtze River valley is usually in the middle or early April. In other areas, when the water temperature reaches 18°C ​​in the morning and the weather forecast has no cold current for 3 consecutive days, the temperature will not play. Drop, you can start production. In the season of production, we must be diligent in observing fish and prevent a large number of abortions.
2.2 Pre-production preparation
2.2.1 Production arrangement of fish nests (artificial insemination, water-free hatching of fertilized eggs can eliminate this step) The fish nests are bestowed with willow root and palm bark, and the fish nests are soaked with 3% lime water for 15 minutes before use. Or cook for 30 minutes, then bundled from the base tied into a bamboo raft on the back, bamboo raft length of 1.5m is better, each fish nest interval is 20 ~ 30cm is better. Natural spawning and fertilization After the parents have spawned, the fish nests are arranged 10-15 cm below the water surface near the spawning pool, and are relatively concentrated. The amount of fish nest is generally suitable for one female per female.
2.2.2 Preparation of spawning pool (this step can be omitted in the artificial insemination) Spawning ponds are sterilized with quick lime in the 8th to 10th days prior to spawning of the broodstock. The amount of 900 to 1200 kg/ha is used to kill the enemy and the wild. Miscellaneous fish. At the same time clear the pond grass, bricks, stones and other debris, so as not to lay eggs on the top of the carp. Clean fresh water will be injected 2~3d after clear ponds. When the water is injected, it should be filtered with dense net. The water depth should be 1.0~1.2m.
2.3 Selection of oxytocin parents Selection of sexually mature parents is the key to increasing productivity, egg production, and quality fry.
Female mature broodstock: The appearance of abdominal swelling, soft, elastic, flipping fish body can be seen in the abdomen depression, reproductive hole ruddy, slightly visible grayish yellow egg.
Male mature broodstock: The abdomen has milky white semen out of the abdomen.
2.4 Oxygen-promoting agents and their commonly used oxytocins include luteinizing hormone-releasing hormone analogues (LRH-A), chorionic gonadotropin (HCG) and fish pituitary (PG). The escalating dose of female silver crucian carp: the pituitary dose is 5-6 mg per kg of fish, and the male carp is halved; or LRH-A 30 μg and HCG 1200 IU are injected per kg of fish and the male carp is halved.
The oxytocic agent was injected with 2 mL per kilogram of fish, and the injection was made with 0.7% physiological saline. The injection site was at the base of the fins of the broodstock. The injection needle was pierced at a 45 angle to the axis of the fish, and the oxytogen was injected into the fish once. Natural spawning and fertilization, the injection time of 13 to 15 pm, spawning time early in the morning to early morning. At this time the environment is quiet and conducive to spawning. Artificial insemination injection time 17 to 18 hours, the effect of time in the morning the next day 6 to 8 hours, is conducive to manual operation.

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