1. Ensure the pure culture of the strains, be careful not to use strains contaminated by other bacteria, and do not connect and cultivate them at close range.
2. Strictly control the number of passages of the bacteria, reduce mechanical damage, and ensure that the vitality of the bacteria is not affected.
3. Appropriate low-temperature storage of strains, low-temperature strains such as mushrooms, shiitake mushrooms, etc., are conducive to preserving the vitality of mycelium at 4°C, and high-temperature strains such as straw mushrooms are conducive to preserving the vitality of mycelium at 16°C.
4. Avoid multiple passages in a single or the same medium; strains should not be used for too long, as over-age strains will age, and aging and degradation are organically linked, and strains with weak viability are prone to degradation.
5. Strains should be rejuvenated regularly, under the conditions of suitable temperature, suitable pH, sufficient oxygen, and no bacteria.
6. Perform spore separation every year to discover excellent strains through sexual reproduction, and consolidate the genetic characteristics of excellent strains through tissue separation.
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