Gastrodia can not self-support, must rely on Armillaria and its symbiotic, in order to get nutrition and reproductive growth. Parasitic bacteria can grow in about 200 species of trees, especially broadleaf forests. In general, the cut tree, bamboo or burned tree, bamboo root, and dead tree fallen in the forest will be derived from Armillaria. Armillaria must be able to thrive under certain humid conditions. The soil moisture content should be no less than 45% to 50%, and the air relative humidity should be 75% to 80%. Armillaria can also be cultivated artificially, and bacteria must be cultured first. The collection of bacterium source: In the Gastrointestinal production area, the thin cord of Armillaria mellea (brown sheath red) was collected, or the young, freshly-opened Armillaria mellea were collected in autumn for tissue separation. After the inoculated species was inoculated, the culture was performed in an environment of 22°C to 26°C. About 7 days is the mother's species. Cultivated species: wheat bran 50%, rice bran 19%, gypsum powder 1%, wood chips 30%, water amount. PH value 6 to 7. The above-mentioned culture materials are mixed into a culture bottle, compacted slightly, autoclaved, inoculated under aseptic conditions, cultured at 25° C. to 26° C. for 15 to 17 days, and overgrown with whole bottle cultivating species. Seed culture: Sawdust (fine eucalyptus) is added with water or 20% potato water to form semi-solid medium. For the production, use a 500-ml flask and add 10 grams of sawdust to each bottle. Add water to wet the unswarwed saw. Continue to add water until the liquid level is 0.5 cm deep. After adding enough water, select the branches of the chestnut tree with finger thickness, cut into 6 to 8 cm long branches, cut the skin with a knife into squamas, insert 2 to 3 bottles, and insert the above semi-solid medium obliquely. In the case of sterile inoculation after autoclaving and cultivation at 25°C for 1 to 2 months, brownish red fungus is grown on the medium and shoots, which is the seed material. Stacking and Mixing: The seed material grown from the semi-solid medium is mixed with a fresh shoot of a chestnut tree with a diameter of 3 cm or less and a length of about 30 cm. The method is: a piece of wood is clamped with a piece of fresh wood, and then covered with a mixture of wood chips and sand (at a volume ratio of 1:1, 50% of water content) to fill the gap and cover it with a thickness of 4 cm. Put a layer of fresh material, according to the same method plus filler, according to this accumulation, you can even put 3 to 4 layers. Note that regular watering is kept moist, and after 1 to 2 months, fresh shoots are basically made of fungus, which becomes a seed material for expanding cultivation. China Agricultural Network Editor
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