Discover our range of Chromogenic Substrates

Explore Our Range of Chromogenic Substrates

 

Chromogenic substrates are colorless, soluble molecules composed of two key components:

  1. A chromophore – a chemical group that, upon enzymatic cleavage, releases color.
  2. A specific enzymatic substrate, typically a digestible carbohydrate.

These compounds are invaluable for enzymatic detection because chromogenic substrates specifically bind to the target enzyme. This interaction enables the enzyme to catalyze the release of the chromophore group, producing an insoluble product with a distinct color that verifies the presence and activity of the enzyme being studied. The intensity of the color produced can be quantified in chromogenic assays used in techniques like Western blot, ELISA, immunohistochemistry, enzymatic assays, and microbial detection in culture media.

Why Use Chromogenic Substrates?

Chromogenic substrates find applications across various fields:

  • Microbiology: They’re utilized in culture media, clinical diagnostics, microorganism identification, and even in the food industry.
  • Biotechnology: These substrates offer a cost-effective method for colorimetric detection, applicable from immunohistochemistry to Western blot and ELISA.

Overview of Chromogenic Substrates

Our New Range of Chromogenic Substrates

At DC Fine Chemicals, we’ve developed a new line of chromogenic substrates. We’ve organized them based on the color they generate, the name of the substrate, any alternative names, their CAS numbers, and the specific enzymes they bind to.

  • Lapis Substrates (Deep Blue)
  • X-Substrates (Blue-Green)
  • Magenta Substrates (Magenta to Lilac)
  • Salmon Substrates (Pink)
  • Other Chromogenic Substrates

 

Classification DCFC Code Substrates Synonyms CAS Enzyme
Lapis Substrates

(Deep Blue)

 125290 5-Bromo-3-indolyl phosphate disodium salt Blue-phos 16036-59-2 Alkaline phosphatase
X-Substrates

(Blue-Green)

 

 125030 5-Bromo-4-chloro-3-indolyl-β-D-cellobioside X-Cellobioside 177966-52-8 β-Cellobiosidase
125000 5-Bromo-4-chloro-3-indolyl caprylate X-Caprylate 129541-42-0 Esterase
125220 5-Bromo-4-chloro-3-indolyl-α-D-galactopyranoside X-α-Gal, X-α-D-Galactoside 107021-38-5 α-Galactosidase
124980 5-Bromo-4-chloro-3-indolyl-α-D-glucopyranoside X-α-Glucoside 108789-36-2 α-Glucosidase
124960 5-Bromo-4-chloro-3-indolyl-N-acetyl-β-D-glucosaminide X-N-Acetyl-β-D-glucosaminide; X-Glucosaminide 4264-82-8 N-Acetyl-β-D-glucosaminidase
Magenta Substrates (Magenta to Lilac)

 

 125020 5-Bromo-6-chloro-3-indolyl-β-D-glucopyranoside Magenta-glucoside 93863-89-9 β-Glucosidase
125010 5-Bromo-6-chloro-3-indolyl-α-D-glucopyranoside Magenta-α-D-glucoside 878495-64-8 α-Glucosidase
124990 5-Bromo-6-chloro-3-indolyl phosphate disodium salt Magenta-phos 404366-59-2 Alkaline phosphatase
124970 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside Magenta-gal 93863-88-8 β-Galactosidase
125240 5-Bromo-6-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt Magenta-GlcA CHA salt, Magenta-gluc CHA salt 144110-43-0 β-Glucuronidase
Salmon Substrates (Pink)

 

 125260 6-Chloro-3-indolyl-α-D-galactopyranoside Salmon-α-gal 198402-61-8 α-Galactosidase
103380 6-Chloro-3-indolyl-β-D-galactopyranoside Salmon-gal 138182-21-5 β-Galactosidase
125250 6-Chloro-3-indolyl-β-D-glucopyranoside Salmon-glucoside 159954-28-6 β-Glucosidase
125230 6-Chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt Salmon-glcA CHA salt, Salmon-gluc CHA salt 138182-20-4 β-Glucuronidase
Other Chromogenic Substrates

(Blackish purple)

 125210 5-Bromo-4-chloro-3-indolyl phosphate disodium salt X-Phosphate disodium salt, BCIP 102185-33-1 Alkaline phosphatase, often in conjunction with NBT
125270 Nitro blue tetrazolium NBT, Nitro BT 298-83-9 Alkaline phosphatase

 

If you’d like to learn more about these substrates and how they could enhance your operations, feel free to reach out to us at contact@dcfinechemicals.com. We’ll be happy to arrange a meeting with one of our team members to discuss further.

 

Article written by Sherry Cacay, Marketing Manager of DC Fine Chemicals

 

Discover Our Chromogenic Substrates

Roughness Profilometer

Roughness Profilometer, also known as surface roughness meter, surface smoothness meter, surface roughness tester, roughness measurement meter, roughness tester, and other names. It has the characteristics of high measurement accuracy, wide measurement range, easy operation, portability, and stable operation. It can be widely used for the detection of various metal and non-metal processing surfaces. This instrument is a pocket instrument that integrates sensors and hosts, with handheld characteristics, making it more suitable for use in production sites. The exterior design is sturdy and durable, with significant resistance to electromagnetic interference, in line with current design trends.

Roughness Profile Measuring Instrument 4

The application fields of roughness meters include:

1. Mechanical processing and manufacturing industry, mainly metal processing and manufacturing. Roughness meters were originally developed to detect the surface roughness of machined parts. Especially, stylus type roughness measuring instruments are more suitable for detecting hard metal surfaces. For example, the automotive parts processing and manufacturing industry, the mechanical parts processing and manufacturing industry, and so on. As long as these processing and manufacturing industries involve the surface quality of workpieces, the detection application of roughness meters is essential.

2. In the non-metallic processing and manufacturing industry, with the progress and development of technology, more and more new materials are applied to processing processes, such as ceramics, plastics, polyethylene, etc. Some bearings are now made of special ceramic materials, and pump valves are made of polyethylene materials. These materials have a hard texture, and some applications can replace metal materials to make workpieces. During production and processing, their surface roughness also needs to be tested.

3. With the continuous strengthening and improvement of the technology and functions of roughness meters, as well as their in-depth promotion and application, more and more industries have been found to require roughness detection. In addition to mechanical processing and manufacturing, roughness evaluation is also required in the production and processing of power, communication, electronics, such as couplings on switches, integrated circuit semiconductors, and even stationery, tableware, and other products used in people's daily lives The surface roughness of human teeth needs to be tested.

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