Discover our range of Chromogenic Substrates

Explore Our Range of Chromogenic Substrates

 

Chromogenic substrates are colorless, soluble molecules composed of two key components:

1. A chromophore – a chemical group that, upon enzymatic cleavage, releases color.
2. A specific enzymatic substrate, typically a digestible carbohydrate.

These compounds serve as valuable tools for enzymatic detection because they specifically bind to the target enzyme. When this reaction occurs, the enzyme catalyzes the separation of the chromophore group, producing an insoluble product with a distinct color that confirms the presence and activity of the enzyme being studied. The intensity of the color can be quantified in chromogenic assays used in techniques like Western blotting, ELISA, immunohistochemistry, enzymatic assays, and microbial detection in culture media.

Why Use Chromogenic Substrates?

Chromogenic substrates find applications across several fields:

• Microbiology: They are employed in culture media, diagnostic tests in clinical labs, microorganism identification, and even in the food industry.
• Biotechnology: These substrates enable colorimetric detection, offering a cost-effective method from immunohistochemistry to Western blotting and ELISA.

Chromogenic Substrates Overview

Our New Range of Chromogenic Substrates

At DC Fine Chemicals, we’ve developed a new line of chromogenic substrates. We’ve categorized them based on the colors they generate, the substrate names, their alternative synonyms, their CAS numbers, and the specific enzymes they bind to.

• Lapis Substrates (Deep Blue)
• X-Substrates (Blue-Green)
• Magenta Substrates (Magenta to Lilac)
• Salmon Substrates (Pink)
• Other Chromogenic Substrates

 

Classification	DCFC Code	Substrates	Synonyms	CAS Number	Enzyme
Lapis Substrates (Deep Blue)	125290	5-Bromo-3-indolyl phosphate disodium salt	Blue-phos	16036-59-2	Alkaline phosphatase
X-Substrates (Blue-Green) Â	125030	5-Bromo-4-chloro-3-indolyl-β-D-cellobioside	X-Cellobioside	177966-52-8	β-Cellobiosidase
	125000	5-Bromo-4-chloro-3-indolyl caprylate	X-Caprylate	129541-42-0	Esterase
	125220	5-Bromo-4-chloro-3-indolyl-α-D-galactopyranoside	X-α-Gal, X-α-D-Galactoside	107021-38-5	α-Galactosidase
	124980	5-Bromo-4-chloro-3-indolyl-α-D-glucopyranoside	X-α-Glucoside	108789-36-2	α-Glucosidase
	124960	5-Bromo-4-chloro-3-indolyl-N-acetyl-β-D-glucosaminide	X-N-Acetyl-β-D-glucosaminide; X-Glucosaminide	4264-82-8	N-Acetyl-β-D-glucosaminidase
Magenta Substrates (Magenta to Lilac) Â	125020	5-Bromo-6-chloro-3-indolyl-β-D-glucopyranoside	Magenta-glucoside	93863-89-9	β-Glucosidase
	125010	5-Bromo-6-chloro-3-indolyl-α-D-glucopyranoside	Magenta-α-D-glucoside	878495-64-8	α-Glucosidase
	124990	5-Bromo-6-chloro-3-indolyl phosphate disodium salt	Magenta-phos	404366-59-2	Alkaline phosphatase
	124970	5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside	Magenta-gal	93863-88-8	β-Galactosidase
	125240	5-Bromo-6-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt	Magenta-GlcA CHA salt, Magenta-gluc CHA salt	144110-43-0	β-Glucuronidase
Salmon Substrates (Pink) Â	125260	6-Chloro-3-indolyl-α-D-galactopyranoside	Salmon-α-gal	198402-61-8	α-Galactosidase
	103380	6-Chloro-3-indolyl-β-D-galactopyranoside	Salmon-gal	138182-21-5	β-Galactosidase
	125250	6-Chloro-3-indolyl-β-D-glucopyranoside	Salmon-glucoside	159954-28-6	β-Glucosidase
	125230	6-Chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt	Salmon-glcA CHA salt, Salmon-gluc CHA salt	138182-20-4	β-Glucuronidase
Other Chromogenic Substrates (Blackish Purple)	125210	5-Bromo-4-chloro-3-indolyl phosphate disodium salt	X-Phosphate disodium salt, BCIP	102185-33-1	Alkaline phosphatase, often in conjunction with NBT
	125270	Nitro blue tetrazolium	NBT, Nitro BT	298-83-9	Alkaline phosphatase

 

If you’re interested in learning more about these substrates and how they can enhance your processes, feel free to reach out to us. Contact us at to schedule a meeting with one of our experts.

 

Written by Sherry Cacay, Marketing Manager of DC Fine Chemicals

 

Discover Our Chromogenic Substrates