Strengthening training wild boars
Patiently domesticating wild boars across fake boars can't be intimidated and quiet. Before harvesting, first cut the long hairs before the wild boar wrapper, so as not to seize the long hair and semen contaminated with semen.
1. The boar's sexual maturity is 12 to 14 months old, and 14 to 15 months old boars are tamed before they are trained for sperm collection.
2. Breeding training for wild boars. Initially using estrus sows to encourage boars to drive and collect sperm, several times before driving a false mother, that is: the urine of the sow and the boar semen, The foreskin liquid is sprinkled on the back of the false mother table, causing the new boar to approach the false mother table and stimulate the new boar to climb onto the false mother table; 14 to 18 months old wild boars collect sperm once a week for more than a year and a half. Semen 2 ~ 3 times is appropriate.
3. In the summer, high temperature should be avoided to affect the performance of semen selection and semen. Install showers, curtains, and air-conditioning equipment in the boar to reduce the temperature and ensure the quality of the semen.
4. Wild boar nutrition should use full-priced diet, pay attention to the supply of good protein, vitamins and minerals.
Semen collection
1. Picking the necessary equipment and utensils. Semen collection using a false mother Taiwan, sperm with a 500 ml wide-mouth glass bottles or vacuum flasks, the fine-mesh gauze or filter cups by high temperature sterilization, wipe the clean towel on the abdomen of the boar, picking the rubber gloves used.
2. Semen collection step.
1 Before the wild boar driving a false mother, first wash the abdomen, and squeeze the foreskin to squeeze out the urine, and then dry it with a towel.
After 2 wild boars ride on the false master platform, the sperm extractor stands on the right side of the false motherboard. After the boar stimulates orgasm and stretches the penis several times, he then grips the penis with gloves or a clean bare right hand. Spiral parts, give appropriate pressure at the right time.
3 The first 5 to 10 ml of clear semen was given to give up (containing bacteria or impurities to prevent contamination of the semen), and then the semen was injected using a wide-mouth plastic bottle or thermos bottle covered with three layers of gauze in the semen. Colloids are filtered. The collected semen is best taken to the laboratory within 5 minutes.
4 In order to avoid rapid drop of semen temperature, the sperm bottle should be placed in the warmer or directly using a thermos bottle to prevent the sperm from being shocked or injured by the temperature change.
3. The original semen treatment. 1 Avoid overheating or undercooling the environment where the semen is located, as the sperm is sensitive to temperature changes. 2 Avoid sperm exposure to water or harmful chemicals. 3 Avoid direct sunlight on semen. 4 Reduce the volume of air exposed to semen. 5 Slowly stir the semen without shaking.
4. Semen quality inspection. Coffee color indicates high sperm density; milk color indicates a decrease in sperm density; yellow indicates semen contains urine or foreskin secretion; red indicates semen contains blood; green indicates that the semen contains pus. Sperm is odorless. A 1 ml volume corresponds to 1 gram of weight.
Semen dilution and preservation
1. Add semen dilution. Its purpose is to increase the pH of the semen concentration, but also to provide sperm with nutrients and to increase sperm motility. Increase the amount of semen to prevent the temperature from reducing the cold strike on the sperm and provide a buffer to prevent it. It is usually diluted with a convenient and readily available 5% to 6% glucose solution. This solution can protect the limited energy available to the sperm to facilitate short-term sperm survival. Dilution of semen with sub-dilution is usually done the same day. It should not be used. For long-term preservation, it is advisable to add an appropriate amount of antibiotics to the diluted semen to inhibit bacterial growth.
2. Diluted and saved. Its purpose is to preserve the sperm's longevity, in addition to increasing the dose of semen. Placed at 15 °C ~ 18 °C can be stored for 3 to 4 days more stable, the purpose is to inhibit the viability of sperm in the storage period, the following is based on different needs and the formula used: 1 room temperature preservation method. The storage temperature is 15°C～20°C, and it can be stored in incubators and thermos; the temperature is suitable for indoor use. 2 cryopreservation method. Storage temperature 8 °C ~ 15 °C, can be stored in well water, etc., the diluent used should be added anti-shock agent yolk. Pay attention to control the temperature in the scope of adaptation, can not suddenly rise and fall.
3. Recipe.
Formula 1: glucose 5g, sodium citrate dihydrate 0.3g, disodium edetate 0.1g, distilled water 100ml, penicillin 100,000 international units, dihydrostreptomycin 1000μg/ml, used for room temperature preservation .
Formula 2: glucose 5 grams, 0.5 grams of sodium citrate dihydrate, distilled water, 100 ml, blue, streptomycin appropriate, used for room temperature preservation.
Group 3: 5 g of glucose, 0.5 g of sodium citrate dihydrate, 100 ml of distilled water, 3 ml of fresh egg yolk, and the appropriate amount of cyanine and streptomycin for normal temperature preservation or normal cryopreservation.
Group 4: 5 g of glucose, 0.3 g of sodium citrate dihydrate, 0.1 g of EDTA, 100 ml of distilled water to form the base liquid, 95 ml of the base fluid, 5 ml of fresh egg yolk, and 40 ml of fresh yolk and streptomycin 100,000 international units for normal temperature preservation or ordinary cold storage.
Formula 5: 5 g of glucose, 0.3 g of sodium citrate dihydrate, 0.1 g of EDTA, 100 ml of distilled water, 7 ml of egg yolk, 100,000 international units of cyanine and streptomycin, used for room temperature preservation or cryopreservation (7°C ~13°C).
Formula 6: glucose 6.00 g, sodium citrate 0.37 g, sodium bicarbonate 0.12 g, EDTA 0.37 g, penicillin 0.5 g, streptomycin 0.5 g, and deionized distilled water was added to 100 ml.
4. Preparation method. Use a balance and a graduated cylinder to weigh the medicine and water, dissolve and sterilize. Add the streptomycin to a temperature of about 30°C, and filter with a sterile gauze layer of 4 or 2 layers of silk or qualitative rapid filter paper. If you use yolk, add yolk and mix thoroughly. Make it ready for use now. Disinfect and rinse the balance pan with a cotton ball at the end of work.
Preparation of Diluted Preservation Solution: 1 Diluted Preservation Solution After the powder is formulated, it is added directly into deionized distilled water heated to 37° C. and mixed and dissolved. 2 The prepared diluted storage solution was stored in an environment of 4° C. The effective time was one week.
5. Semen dilution.
1 The semen should be diluted as soon as possible within 30 minutes. When the semen is diluted, the semen is first lowered to 20°C to 25°C at room temperature. The temperature of the diluent should be similar to the temperature of the semen.
2 Dilutions slowly pour into the semen flask along the tube wall and shake gently. Diluted semen is allowed to cool at room temperature to 20°C～25°C, and then put in the refrigerator. In general, every 12 hours Gently shake once to prevent sperm from falling to the bottom.
3 semen dilution factor, depending on the sperm concentration, the general preservation of semen with a sperm count of 200 million per ml is good, this high concentration of semen should be added to do the deferral when adding isothermal dilution to achieve sufficient insemination The injection volume is generally 100 ml.
4 When the dilution ratio is greater than 1:3, add half of the dilution at the beginning, and after 10 minutes, add the other half of the dilution.
6. Preserve equipment and utensils for semen. 16 °C ~ 18 °C constant temperature refrigerator, semen bottles.
7. Semen transport. Should be full, without air, try to shorten the transport time, to prevent vibration and temperature changes, control the temperature at 15 °C ~ 20 °C or 8 °C ~ 15 °C (depending on the preservation method used in the dilution and boar semen characteristics).
Insemination
Required equipment: vas deferens, sterilizer (used to sterilize vas deferens), refrigerated box (can maintain the temperature in the box at 16 °C ~ 17 °C), storage cabinet (store clean vas deferens), deionizer, high and low thermometer, knife.
1. Remove the semen from the constant temperature refrigerator and slowly heat it in a water bath to 25°C to 36°C.
2. Wash sows' vulva. Wash the sows' vulva with warm water and wipe dry.
3. Insulin timing. Generally 18 to 72 hours after estrus, old sows are appropriately advanced and can lose sperm in the afternoon of the same day. After the middle-aged sow is inseminated on the second day after estrus, the primiparous sow is appropriately pushed back and inseminated on the third day after estrus. After the weaning sow 3 to 6 days after weaning, the estrus appeared 6 to 12 hours after the standing reaction to perform the first insemination and breeding; the gilts and the sows in the estrus that were more than 7 days after the weaning had estrous response to standing. , to carry out breeding (insemination). Two insemination interval 12 to 24 hours, each instinct instinct 2 to 3 times.
4. Press the sow's back with a sandbag. Insert the insemination gun into the sow's genital tract at 45 degrees upwards. When the vas deferens enters about 10 centimeters, when you feel resistance, make the vas deferens level and continue inserting slowly until you feel it. The front end of the vas deferens is locked (slightly pulled back). If there is a feeling of lock, it means that it has reached the cervix mouth (insertion depth 25 to 35 centimeters, miniature pigs 13 to 25 centimeters), and is inserted at the end of the uterus. Intrauterine insemination should be performed as far as possible. Then slowly shake the semen, cut the mouth of the semen bag with scissors, and connect it to the vas deferens to keep the semen bag upright and keep the flow of the semen open.
5. In the process of insemination, try to avoid the insemination method using forceful extrusion. When insemination is difficult, the uterus can contract to produce negative pressure by touching the sows' udder or vulva and pressing the back to stimulate the sow. The semen is absorbed; if the semen is still difficult to input, it may be that the vas deferens is inserted too far into the uterus, and the vas deferens need to be pulled up.
6. The insemination time should be at least 3 to 5 minutes. After a sow is fed, the back end of the vas deferens should be folded to keep it in the sow's reproductive tract 3 to 5 while preventing the air from entering the sow's reproductive tract. Minutes later, remove the insemination gun in a clockwise direction and remove the sandbag.
7. Measures to prevent backflow of semen. The insertion site is accurate and inserted into the uterus; the massage is done at the nest, the clitoris or the vulva; the semen is slowly entered at a slow speed and the vas deferens stays in the uterus for a period of time; the dripping sperm is on the nose of the sow.
8. Cleaning and disinfection of equipment.
1 Wash: Wash with 2% sodium bicarbonate (baking soda). Rinse again with clean water 5-6 times.
2 Disinfection: Glass, metal, gauze, towel Sterilized by dry heat (120°C for 15-30 minutes) in a thermostatic oven; glass and metal are best dried after boiling; gauze is best to dry after boiling: The fine rubber hose is boiled or boiled or steam sterilized; the water thermometer is sterilized with an alcohol swab; the dilute solution is boiled for 10 to 15 minutes; the sterilized device is rinsed with diluent before use.
3 Operation Room Hygiene: The window is clear and the console and the floor need to be wiped every day. The operator should wear white overalls to make it dust-free, fly-free and sterile. Smoking is strictly prohibited. Instruments and veterinary equipment and drugs must not be used together and mixed together.
The artificial insemination technology of wild boar was tested in Wushan Huangshanzhen Animal Husbandry Co., Ltd., 200 wild sows symbiotically 192 litters and 2021 wild piglets, the quasi-tyre rate was 96%, with an average of 10.52 heads/nest, indicating that special wild boar artificial insemination not only It is feasible and worth learning, and it can make full use of the good traits of boars to provide services for boar production.
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