Founded in 1989, BMG LABTECH is a leading developer and manufacturer of microplate readers, offering many measurement methods and integrated liquid handling equipment. BMG LABTECH has a number of pioneering records in the field of microplate reader reading technology: the first multi-function microplate reader including FP, the first laser turboscope for 384-well plates, the first Combined microplate reader with high-performance chemiluminescence and the first compact fluorescence reader with integrated pipette system for cell-based analysis and the first integration Multi-function microplate reader for UV/Vis absorbance detection. To meet customer needs in life sciences, proteomics, genomics, drug discovery and high-throughput screening (HTS), BMG LABTECH has developed a plate reader solution for analysis. They are used to read fluorescence polarization (FP), fluorescence intensity (FI), time-resolved fluorescence immunoassay (TRF), ie HTRF®, continuous and transient chemiluminescence (L), AlphaScreenTM, absorbance (ABS) and turbidity measuring. All instruments offer the highest sensitivity, flexibility and reliability and are easy to integrate into automation systems.
Below we list the top 10 applications of BMG global users for BMG microplate readers:
ORAC (oxygen antioxidant capacity) analysis - FLUOstar OPTIMA (AN-148)
ORAC analysis is the most common method for analyzing the antioxidant capacity of a substance. With the help of FLUOstar OPTIMA's high-precision fluorescence detection and its easy-to-use software, the antioxidant properties of the substance to be tested can be directly calculated from the Trolox® standard curve. Each step of the reaction can be performed in real time in real time through the preset program of the instrument. Moreover, the use of built-in reagent injectors ensures consistency and repeatability of test results.
AN-148: http://
DNA quantification - FLUOstar / POLARstar Galaxy (AN-116)
The most common method of detecting DNA concentration is to detect the light absorption of DNA at 260 nm (A260). The microplate reader used for this assay must be equipped with a short UV optical system (bottom and bottom). BMG FLUOstar and POLARstar Galaxy have this feature, they are equipped with 280-12nm and 260-12nm filters as standard for DNA quantification. Multi-wavelength light absorption analysis is also possible.
Bradford Method Protein Quantification - FLUOstar Omega
Determining the protein concentration in a sample is a necessary and commonly used method in biochemistry, and there are currently a number of different colorimetric protein quantification methods. The most commonly used are the Bradford method, the Lowry method and the BCA method. BMG FLUOstar Omega's spectrometers fully meet the needs of these assays. The full wavelength absorption spectrum of one sample can be determined in one second. Moreover, the unique features of the Omega Evaluation software allow you to get maximum and minimum absorbance by simply clicking on the absorption spectrum. You can also choose any wavelength, and the software will automatically give the optical density value in any hole. Ultra-fast detection speed and easy-to-use operating software ensure unmatched flexibility of the instrument, making it easy to optimize different samples.
AN-116: http://
Fluo-8 calcium release analysis - NOVOstar (AN-157)
The determination of receptor activation can be achieved by measuring intracellular calcium ions, that is, the method can be used to screen for specific agonists or antagonists. There are two key factors for accurate analysis of intracellular calcium:
1. Ability to rapidly detect changes in calcium ions after introduction of agonists/antagonists into cells
2. Choose a dye that permeable to the membrane and combined with calcium ions to greatly increase the signal
BMG NOVOstar's unique syringe system injects a very small amount (<1 μL) of agonist/antagonist from the reagent plate into the test plate and initiates the test mode at any time (before, during, and after injection) ( Fluorescence intensity, luminescence, light absorption or fluorescence polarization make NOVOstar a perfect cell-based rapid kinetic analysis that fully meets the requirements of 1.
ABD Bioquest's Screen QuestTM Fluo-8 NW calcium kit meets the requirements of 2. Fluo-8 NW is a permeable membrane dye that combines with calcium ions to increase the fluorescence intensity by a factor of 100, more than twice that of Fluo-4 NW, and has the same calcium ion as Fluo-4 NW. Affinity.
Therefore, the combination of NOVOstar and Fluo-8 NW is the perfect platform for intracellular calcium ion determination.
AN-157: http://
P450-GloTM Analysis - FLUOstar OPTIMA (AN-151)
P450-GloTM analysis provides a fast, sensitive and highly reproducible method for P450 screening. The system is compatible with traditional P450 analysis protocols in automatic or manual multi-microplate mode.
The BMG FLUOstar OPTIMA Microplate Reader provides the perfect inspection platform for the Promega P450-GloTM analytical method. Fluostar OPTIMA uses its proprietary self-illumination detection system to minimize background signal and, therefore, increase data confidence. At the same time, combined with the built-in 2 reagent syringes (optional) and the standard 45 ° C incubator, FLUOstar OPTIMA makes it easy to meet all your P450 screening needs. Not only FLUOstar OPTIMA, BMG LABTECH's LUMIstar OPTIMA, POLARstar OPTIMA and PHERAstar can be used for Promega's P450-GloTM analysis. In fact, PHERAstar combined with the DeeracTM liquid operating system has successfully transformed P450-GloTM analysis into a miniaturized high-throughput screening platform.
AN-151: http://
Light Absorption of DNA - POLARstar Omega (AN-168)
The POLARstar Omega standard spectrometer is very convenient for the determination of DNA light absorption - single wavelength (260 nm) determination or full wavelength measurement including maximum absorption peak. Moreover, with the help of the new version of the analysis software MARS, the nucleic acid concentration can be determined based on the extinction coefficient.
POLARstar Omega makes it easy to simultaneously determine the A260/A280 value characterizing the purity of a DNA sample when assayed at A260. The 220m-850nm wavelength absorption spectrum that can be completed in one second per well helps to determine the purity of the DNA sample.
AN-168: http://
NADH/NADPH Detection - Omega Series Fast, Full Wavelength Spectrometer (AN-170)
FLUOstar, POLARstar and SPECTROstar Omega microplate readers come standard with UV/Vis spectrometers for light absorption in the 220nm-850nm wavelength range with 1, 2, 5 and 10nm resolution. The detection speed is holes/second. Thanks to the flexible function of the Omega series of instruments, the light absorption detection function that cannot be realized on many multi-function micro-plate readers can be realized on the same instrument. Absorption spectroscopy of cofactors such as NAD+, NADH, NADP+, and NADPH.
AN-170: http://
ORAC (oxygen antioxidant capacity) analysis - POLARstar Omega / PHERAstar FS (AN-197)
Both POLARstar Omega and PHERAstar FS are equipped with built-in reagent syringes for easy ORAC analysis and are compatible with different flux microplates such as 96 and 364. The data obtained from 96-well and 384-well plates are very similar. In PHERAstar FS, the sample volume can be further reduced.
MARS is the standard software for every Omega instrument and PHERAstar and can be used for automatic calculation of antioxidant capacity. The preset template combined with the curve scaling feature makes ORAC results extremely fast and easy.
AN-197: http://
Cell migration - POLARstar Omega (AN-204)
POLARstar Omega has a variety of cell detection capabilities, including cell proliferation assays, cell viability assays, toxicity assays, intracellular pH, and apoptosis. It also has detection capabilities for fluorescent proteins, second messengers such as cAMP and Ca2+, and reporter-based expression systems. POLARstar Omega has important features for enhanced cell analysis, such as bottom-reading for the determination of adherent cells, optional gas control module for experimental conditions such as 5% CO2, built-in reagent injectors, and in-hole scanning function determination Fluorescence intensity at different locations. Moreover, the in-hole scanning function ensures a more accurate measurement of cell samples that grow unevenly.
AN-204: http://
AlphaLISA® Analysis - PHERAstar Plus (AN-190)
The IgG AlphaLISA assay kit can be successfully applied to PHERAstar Plus. In a 384-well standard volume assay plate, the sensitivity of the assay reached the nominal value of the kit manufacturer. In the micro volume detection plate, the detection sensitivity is slightly weakened. Nonetheless, in PHERAstar Plus, the analytical volume can be as small as 17 μL with good sensitivity and good Z values.
In the 384-well micro-volume detection mode, the multi-function high-throughput microplate reader PHERAstar Plus exhibits excellent performance in AlphaScreen and AlphaLISA detection functions. Easy-to-use control software makes it easy for users to optimize based on detection sensitivity and number of readings. PHERAstar Plus is a microplate reader specifically designed to meet all high-throughput detection modes (fluorescence intensity, reagent-resolved fluorescence, fluorescence polarization, self-luminescence, AlphaScreen, and light absorption) and up to 1536 wells of all types of microplates.
AN-190: http://
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