The HA tag system utilizes a HA (influenza hemagglutinin epitope: YPYDVPDYA) short peptide peptide to be fused to the target protein. The HA tag can be located at the C-terminus or N-terminus of the protein. This system has been widely used in a variety of cell types, and the corresponding HA-tagged antibodies are also widely used. HA-tagged antibodies specifically recognize the HA-tagged fusion protein at the C-terminus or N-terminus, and can also be used for detection and expression of HA-tag fusion proteins, intracellular localization, and purification, qualitative or quantitative detection of HA. Tag fusion expresses proteins and the like.
Factors to consider when selecting the appropriate HA-tagged antibody
When choosing a label antibody, we mainly rely on the needs of our own experimental application. Factors to be considered here include the type of application of the HA antibody, the clonality of the HA antibody, and the source of preparation. For example, can the HA antibody be used for the detection of immunofluorescence IF? Can it be used in Western Bloting and immunoprecipitation IP experiments? Do you need HRP-conjugated monoclonal HA-tagged antibodies? Is it better to have a rabbit-derived HA polyclonal antibody?
In general, the more types of applications, the greater the choice in use, and the monoclonal antibodies from mouse sources are generally superior in specificity and stability to polyclonal antibodies, and polyclonal. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl HA antibody (if the dilution ratio of WB detection is 1:5000, the final use solution is equivalent to 500 ml), 1 ml of HA antibody with lower potency (if the dilution ratio of WB test is 1:200) The final use solution is equivalent to 200ml). Therefore, when selecting antibodies, not only the amount of antibody needs to be considered, but also the titer of the antibody, that is, the recommended dilution ratio for different applications.
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| Product name and description | Brand | Item number | product manual |
| Anti-HA Tag Mouse Monoclonal Antibody (4F6) | Abbkine | A02040 | Details |
| HRP Conjugated Anti-HA Tag Monoclonal Antibody (4F6) | Abbkine | A02045 | Details |
| Anti-HA Tag Rabbit Polyclonal Antibody | Abbkine | A02041 | Details |
Why choose Abbkine's HA monoclonal antibody (Cat. No. A02040)?
The HA antibodies currently available on the market are either domestically packaged or manufactured; they are also imported, such as Thermo Pierce, Abcam, Sigma, Millipore, etc. Domestically-packaged HA antibodies are insufficient in quality stability, and after aliquoting, their potency is also lost. Some of the HA antibodies originating in domestic laboratories have good detection activity, but there are gaps in the application type, and it is difficult to control the stability between batches, and only a small amount of domestically produced antibodies are also available in China. Polyclonal antibody. Among the imported brands, Thermo Pierce's Anti-HA Epitope Tag Monoclonal Antibody antibody is used more, but the price is very high. Similarly, Abcam's four-star recommended HA tag antibody [HA.C5] (ab18181) antibody is also the price. Millipore's Anti-HA Tag (05-904) is a 200μl package and is worth considering in terms of price/performance.
If you only want to buy a HA-labeled antibody that is more cost-effective and also an imported brand, then Abbkine may be one of your important considerations. We recommend Abbkine's HA monoclonal antibody Anti-HA Tag Monoclonal Antibody (4F6) (Cat. No. A02040). The immunogen used for the monoclonal HA antibody is a KLH-conjugated influenza virus hemagglutinin epitope HA (YPYDVPDYA) synthetic polypeptide, which recognizes HA-tagged recombinant proteins overexpressed in cells, including HA-recombinant proteins at the amino acid and carboxy terminus. . Because they are all derived from the same monoclonal cell line, the antibody products of Pierce and Abcam are actually the same product, but the price is only 1/5 and 1/3 of their respective. The price/performance ratio is really obvious. As a production brand in the United States, Abbkine provides OEM services to a number of internationally renowned brands, so its price is low and its quality has undergone market testing.
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| Product name and description | Brand | Item number | product manual |
| Anti-HA Tag Mouse Monoclonal Antibody (4F6) | Abbkine | A02040 | Details |
| HRP Conjugated Anti-HA Tag Monoclonal Antibody (4F6) | Abbkine | A02045 | Details |
| Anti-HA Tag Rabbit Polyclonal Antibody | Abbkine | A02041 | Details |
The antibody can be used in Western Bloting, immunocytochemistry and immunofluorescence ICC/IF; during use, there are customer reports that can also be used for immunoprecipitation IP and chromosome immunoprecipitation ChIP experiments, etc., of course, depending on the Experiment to determine. In addition, Abbkine also provides HRP-conjugated HA-tagged antibodies, especially for IP-WB verification to remove secondary antibody interference; rabbit-derived polyclonal HA antibodies also provide customers with another HA-label detection protocol.
I used Abbkine's antibodies because they gave me a trial, free of charge, and started the WB experiment. The effect is ok. Once I was in a hurry to do an IP experiment, I couldn't find a suitable spot antibody at the moment, so I tried it and the result was really made. - Sun Yat-Sen University Medical Department customer
Abbkine also offers 1ml large packaging and larger custom-designed HA-labeled antibodies, making it ideal for routine laboratory testing of HA-labeled systems. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.
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| Product name and description | Brand | Item number | product manual |
| Anti-HA Tag Mouse Monoclonal Antibody (4F6) | Abbkine | A02040 | Details |
| HRP Conjugated Anti-HA Tag Monoclonal Antibody (4F6) | Abbkine | A02045 | Details |
| Anti-HA Tag Rabbit Polyclonal Antibody | Abbkine | A02041 | Details |
The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its China. 
The regional agent will provide customers with the most cost-effective routine immunological reagents, such as various tag antibodies, internal reference antibodies, conventional primary antibodies and a full range of secondary antibodies, antibody detection substrates and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.
Shenzhen Guangyang Zhongkang Technology Co., Ltd. , https://www.szlighttherapymachine.com