The cultivation of seaweed dishes in Zhejiang sea area is the largest in the country. At present, the most important reason for influencing cultivation is seedlings. The quantity and quality of seedlings can not meet the needs of cultivation. The artificial breeding of Sargassum fusiformis has been around for about 10 years, but there are still some technical problems that have caused the amount of artificial breeding of Sargassum fuji in recent years to fall short of the proper production scale. The test work over the past few years is summarized as follows:
1. Selection, collection and treatment of vegetables: Choose mature vegetables with good quality. Dry the vegetables in the room for 3 to 8 hours. The dehydration rate is 65 to 75%. According to the maturity of the vegetables to determine the collection time, there are certain differences in the maturity of the different sea areas. In the sea area of ​​Zhejiang Province, it is generally possible to collect mature vegetables from June to August. There are 2 to 4 batches of Hijiki during the tidal period. The quality of vegetables is an important factor affecting the yield of nursery.
2. Picking seedlings: Proper dryness is conducive to the release of fertilized eggs and removal of some of the algae. There are two methods to collect seedlings: dispersal seedlings and seedlings: In accordance with the area of ​​the attachment base, the treated algae are dispersed on the water, and the eggs are fertilized and released to spread the fertilized eggs (mycoplasma) uniformly. At the bottom of the attachment base. Collecting fertilized eggs and collecting seedlings: Concentrate the seedlings in several nursery ponds, inflate and regularly stir, collect the fertilized eggs (protozoa) released by the algae with a 200-mesh sieve, collect the young sporozoites and count, wash the young spores. According to the area of ​​the attachment base, the young sporophyte body is evenly spread on the nursery pond with the attachment base on the bottom. The 200-mesh sieve has a good collection effect. The percentage of adhesion was affected by the degree of shade, the time of seedling collection, and the conditions of light and temperature. Suitable conditions are light illumination above 1000Lx, temperature around 24°C, and young spore body attachment density of 20-40 trees/cm2. Comparing the above two methods, the method of collecting fertilized eggs can remove most of the predators that are attached to the algae, such as algae and hook-fish, which is conducive to the normal cultivation and growth of the seedlings. The seedlings were collected at the beginning of June. By the end of June, the visible density of young sporophytes was 16/cm2. The growth is almost normal. Algae should be collected in time. Since the eggs have basically been released, this kind of algae cannot be used as a second seedling. The rainy days when picking seedlings have a certain influence on the ability of the young sporophytes that separate from the algae to adhere to the attachment base.
3. Attachment base test: Select four attachment bases, including four attachment bases for Dacron cloth, chemical fiber cloth, bamboo blinds, and rock reefs. The test procedure is the same as above. The attachment of young sporophyte at the initial stage is not different, and the seedling culture process is in progress. The effect on growth is also not significant. There is a certain difference in the formation and attachment of pseudosporum of young sporophytes. Rock and reefs are common, and indeed good fabrics are poor. The chemical fiber cloth is next, and it is better as a bamboo screen with a kelp seedling applicator. However, the bamboo screen treatment is not thorough, and the leachate in the cultivation process affects the water quality. In addition, the bamboo green is denser and harder, and the false root is hardly firm. Attached. It is better to use bamboo white as a seedling applicator.
4. Indoor breeding and management: Indoor breeding is the key to the entire artificial breeding. In three phases, the natural light and temperature of the single-cell algae cultivation room were used in the initial stage, and the seawater used for culture was subjected to bleaching treatment and 1/2 nutrient solution was added. In the mid-season cultivation in the kelp nursery room, dimming, natural water temperature, plus nutrient solution. Later in the kelp nursery room for cultivation, dimming, low temperature water with kelp breeding seedlings to reduce the temperature. For different indoor cultivation periods, the conditions of light, temperature and fertilization were changed accordingly.
5. Seedlings and vegetables cryopreservation test: The main temperature test, cryopreservation 0 °C and cooling stored at about 25 °C. The cryopreservation effect is good and will not rot in 15 days. Room temperature is about 25°C, and it starts to rot in 10 to 20 days. However, fake roots can grow new seedlings. Ten days later, new seedlings start to rot. The seedlings were kept at a low temperature of 0 to 4°C. After 30 days, the seedlings grew normally. Management during the preservation period includes changing water every day, inflating or running water, and low light. Seedlings and vegetables can be preserved at low temperatures for a long time. This is an important method. It can remove some of the attached algae, increase the ability to adhere, increase the quality of seedlings, and regulate the cultivation time.
6. Fake root summer training autumn seedlings: after cultivation of terrapins on the cultivated truss in the sea area, false roots are trapped in the net rope to remove the algae, and several pieces are knitted into bundles. The net rope is hung on a cultivation truss in the sea area. Sea area test: The experiment was conducted at two points. One test site had less wind and waves, and the transparency was greater. The other test site had larger waves and less transparency. Observe the growth status and sea state during the cultivation period. The trials with large winds and waves in the open sea are better, with 1 mu producing 1.6 mu of seedlings. Water depth culture test: The above-mentioned net ropes were hung on different water depth cultivars, and the growth status and sea state during cultivation were observed. Differences in different water depths are not significant.
7. Preparation and culture of indoor cells and tissue seedlings: Hormone treatment experiments were conducted with auxin (LAA, NAA), gibberellin (GA) and other plant growth hormones, supplemented by the addition of various nutrient salts. Observe the results of the culture. Culture tests from different sources: For cells and sections of algae (hybrid roots, primary leaves, secondary leaves, stems) in different physiological conditions, the relevant nutrients were added. The trial is in progress. The cultivation of Qiugenduxia in autumn seedlings, indoor cells and tissue seedlings is a promising approach.
1. Selection, collection and treatment of vegetables: Choose mature vegetables with good quality. Dry the vegetables in the room for 3 to 8 hours. The dehydration rate is 65 to 75%. According to the maturity of the vegetables to determine the collection time, there are certain differences in the maturity of the different sea areas. In the sea area of ​​Zhejiang Province, it is generally possible to collect mature vegetables from June to August. There are 2 to 4 batches of Hijiki during the tidal period. The quality of vegetables is an important factor affecting the yield of nursery.
2. Picking seedlings: Proper dryness is conducive to the release of fertilized eggs and removal of some of the algae. There are two methods to collect seedlings: dispersal seedlings and seedlings: In accordance with the area of ​​the attachment base, the treated algae are dispersed on the water, and the eggs are fertilized and released to spread the fertilized eggs (mycoplasma) uniformly. At the bottom of the attachment base. Collecting fertilized eggs and collecting seedlings: Concentrate the seedlings in several nursery ponds, inflate and regularly stir, collect the fertilized eggs (protozoa) released by the algae with a 200-mesh sieve, collect the young sporozoites and count, wash the young spores. According to the area of ​​the attachment base, the young sporophyte body is evenly spread on the nursery pond with the attachment base on the bottom. The 200-mesh sieve has a good collection effect. The percentage of adhesion was affected by the degree of shade, the time of seedling collection, and the conditions of light and temperature. Suitable conditions are light illumination above 1000Lx, temperature around 24°C, and young spore body attachment density of 20-40 trees/cm2. Comparing the above two methods, the method of collecting fertilized eggs can remove most of the predators that are attached to the algae, such as algae and hook-fish, which is conducive to the normal cultivation and growth of the seedlings. The seedlings were collected at the beginning of June. By the end of June, the visible density of young sporophytes was 16/cm2. The growth is almost normal. Algae should be collected in time. Since the eggs have basically been released, this kind of algae cannot be used as a second seedling. The rainy days when picking seedlings have a certain influence on the ability of the young sporophytes that separate from the algae to adhere to the attachment base.
3. Attachment base test: Select four attachment bases, including four attachment bases for Dacron cloth, chemical fiber cloth, bamboo blinds, and rock reefs. The test procedure is the same as above. The attachment of young sporophyte at the initial stage is not different, and the seedling culture process is in progress. The effect on growth is also not significant. There is a certain difference in the formation and attachment of pseudosporum of young sporophytes. Rock and reefs are common, and indeed good fabrics are poor. The chemical fiber cloth is next, and it is better as a bamboo screen with a kelp seedling applicator. However, the bamboo screen treatment is not thorough, and the leachate in the cultivation process affects the water quality. In addition, the bamboo green is denser and harder, and the false root is hardly firm. Attached. It is better to use bamboo white as a seedling applicator.
4. Indoor breeding and management: Indoor breeding is the key to the entire artificial breeding. In three phases, the natural light and temperature of the single-cell algae cultivation room were used in the initial stage, and the seawater used for culture was subjected to bleaching treatment and 1/2 nutrient solution was added. In the mid-season cultivation in the kelp nursery room, dimming, natural water temperature, plus nutrient solution. Later in the kelp nursery room for cultivation, dimming, low temperature water with kelp breeding seedlings to reduce the temperature. For different indoor cultivation periods, the conditions of light, temperature and fertilization were changed accordingly.
5. Seedlings and vegetables cryopreservation test: The main temperature test, cryopreservation 0 °C and cooling stored at about 25 °C. The cryopreservation effect is good and will not rot in 15 days. Room temperature is about 25°C, and it starts to rot in 10 to 20 days. However, fake roots can grow new seedlings. Ten days later, new seedlings start to rot. The seedlings were kept at a low temperature of 0 to 4°C. After 30 days, the seedlings grew normally. Management during the preservation period includes changing water every day, inflating or running water, and low light. Seedlings and vegetables can be preserved at low temperatures for a long time. This is an important method. It can remove some of the attached algae, increase the ability to adhere, increase the quality of seedlings, and regulate the cultivation time.
6. Fake root summer training autumn seedlings: after cultivation of terrapins on the cultivated truss in the sea area, false roots are trapped in the net rope to remove the algae, and several pieces are knitted into bundles. The net rope is hung on a cultivation truss in the sea area. Sea area test: The experiment was conducted at two points. One test site had less wind and waves, and the transparency was greater. The other test site had larger waves and less transparency. Observe the growth status and sea state during the cultivation period. The trials with large winds and waves in the open sea are better, with 1 mu producing 1.6 mu of seedlings. Water depth culture test: The above-mentioned net ropes were hung on different water depth cultivars, and the growth status and sea state during cultivation were observed. Differences in different water depths are not significant.
7. Preparation and culture of indoor cells and tissue seedlings: Hormone treatment experiments were conducted with auxin (LAA, NAA), gibberellin (GA) and other plant growth hormones, supplemented by the addition of various nutrient salts. Observe the results of the culture. Culture tests from different sources: For cells and sections of algae (hybrid roots, primary leaves, secondary leaves, stems) in different physiological conditions, the relevant nutrients were added. The trial is in progress. The cultivation of Qiugenduxia in autumn seedlings, indoor cells and tissue seedlings is a promising approach.
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