Identification
(1) Take 2g of this product powder, add 10ml of ethanol, shake for 20 minutes, absorb supernatant 1ml, steam dry, and add 1 drop of sulfuric acid to the residue, first appear yellow, then turn red and purple, and finally brown .
(2) Take this product powder 2g, add ethanol 20ml, heat reflux for 40 minutes, take the supernatant 10ml, add hydrochloric acid 1ml, heat reflux for 1 hour and then concentrate to about 5ml, add water 10ml, shake with benzene 20ml, extract The mixture was evaporated to dryness and 2 ml of benzene was added to dissolve the residue as a test solution. Another saponin reference substance was taken and benzene was added to make a solution containing 5 mg per 1 ml as the reference solution. According to the thin layer chromatography (Appendix VI B) test, absorb 7μl of each of the above two solutions, and place them on the same silica gel G thin plate, and use benzene-acetone (9:1) as the developing agent, expand, remove, and dry. , sprayed with 8% vanillin anhydrous ethanol solution and sulfuric acid solution (7 → 10) mixture (0.5:5), heated to 100 °C spot color clear [3] clear. In the chromatogram of the test sample, the spots of the same color appear on the positions corresponding to the chromatogram of the reference substance.
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